INTRODUCTION: Fluorescence in situ hybridization (FISH) uses fluorescent molecules to “paint” genes or chromosomes. Download Groove Theory Groove Theory Zip more. This technique is for gene mapping, identification of chromosomal abnormalities and identification of cultured/uncultured microorganisms in environment. Art After Philosophy Kosuth Pdf more. FISH involves the use of short sequences of single-stranded DNA (probes) which are labeled with fluorescent tags, to hybridize, or bind, to the complementary DNA to see the location of those sequences of DNA under the fluorescent microscope. Slide 7: Gene/locus specific probesused to detect the presence absence or location of a particular gene, both in metaphase and interphase cellsCentromere probes designed to hybridize to alpha satellite repeat regions in centromeres, fluoresce brightly due to large number of repeats in centromeres useful for determining the number of copies of a particular chromosomeWhole chromosome paint probesmade from flow-sorted or microdissected chromosomes used to determine composition of marker chromosomes, confirm the presence of chromosome rearrangements FISH PROBES. Slide 25: FISH using r-RNA targeted probes is the method of choice for all studies in which exact cell numbers and cellular locations need to be determined.Development & design of more r-RNA targeted probes for novel microorganisms in environmentThe methodology is being continuously improved so far however, microscopic analysis by FISH has not been automated sufficiently which would be desirable in many investigationsAccurate quantification still remains a challenging task and study needs careful controls.

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